ビシン

Bicine (N,N-bis(2-hydroxyethyl)glycine) is an organic zwitterionic buffer compound belonging to the Good's buffers family. This white crystalline powder exhibits excellent water solubility and maintains stable pH in biochemical systems. Its molecular structure contains both carboxylate and hydroxyl groups, allowing it to function effectively as an amphoteric buffer. Bicine is particularly valued for its minimal metal ion interference and stable pH range (7.6-9.0), making it suitable for sensitive biological applications. The compound's low ultraviolet absorbance and minimal enzyme inhibition contribute to its widespread use in protein research and molecular biology techniques.

What is bicine used for?

Bicine serves as an effective buffer in various biochemical and molecular biology applications, particularly in protein electrophoresis and chromatography. It's commonly employed in plant cell culture media and enzyme assays where traditional buffers may interfere. The compound finds special utility in crystallography studies of metalloproteins due to its low metal-chelation properties. Bicine-based buffers are also used in nucleic acid hybridization procedures and as a component in some diagnostic kits. Its stable buffering capacity at slightly alkaline pH makes it valuable for maintaining physiological conditions during cell fractionation and organelle isolation procedures.

What are the benefits of bicine?

Bicine offers several advantages including exceptional buffering capacity in the pH 7.6-9.0 range with minimal ionic strength effects. It shows negligible interference with biochemical reactions and exhibits low UV absorbance, enabling spectrophotometric analysis. The buffer causes minimal metal ion chelation compared to alternatives like Tris, making it ideal for metalloprotein studies. Bicine demonstrates excellent temperature stability and maintains consistent pH across various experimental conditions. Its zwitterionic nature reduces membrane permeability, minimizing cellular stress in biological systems. These properties combine to make it a superior choice for sensitive protein work and long-duration experiments requiring pH stability.