Welcome to Tangshan Moneide Trading Co., Ltd.
Moneide Chemicals
Tel: 86-315-8309571
WhatsApp/WeChat/Mobile: 0086-15633399667
Skype: janet-honest
Mail: sales@moneidechem.com
Address: 2-7-523 Jidong Building Materials Tangshan, Hebei 064000 China
High Purity Bicine CAS 150254 China Suppliers and Factory
Bicine (N,N-bis(2-hydroxyethyl)glycine) is a premium organic zwitterionic buffer compound from the renowned Good's buffers family, engineered for high-precision biochemical environments. This white crystalline powder is specifically designed to maintain stable pH levels in sensitive biological systems, offering exceptional water solubility and an amphoteric nature derived from its integrated carboxylate and hydroxyl groups.
Highly valued in advanced molecular biology and protein research, Bicine operates effectively within a pH range of 7.6 to 9.0. Its chemical structure ensures minimal interference with metal ions and negligible enzyme inhibition, making it an indispensable tool for protein electrophoresis, chromatography, and the complex crystallography studies of metalloproteins where traditional buffers often fail.
Detailed Parameters
| Chemical Name | Bicine | CAS No. | 150-25-4 |
|---|---|---|---|
| Molecular Formula | C6H13NO4 | EINECS No. | 205-755-1 |
| Molecular Weight | 163.17 | Appearance | White crystalline |
| Assay | 99.0% min. | Water-solubility | Colorless, transparent |
| Water Content | 0.5% max. | pH Range | 4.3 ~ 5.3 |
Key Advantages
Stable pH Buffering
Maintains a consistent physiological pH between 7.6-9.0 with minimal ionic strength effects.
Temperature Stability
Demonstrates excellent temperature stability, ensuring consistent results across varying conditions.
Low Metal Chelation
Minimal metal ion interference compared to Tris, ideal for sensitive metalloprotein studies.
Low UV Absorbance
Negligible interference in spectrophotometric analysis, facilitating clear UV absorbance data.
Zwitterionic Nature
Reduced membrane permeability minimizes cellular stress in delicate biological systems.
Enzyme Compatibility
Exhibits minimal enzyme inhibition, ensuring the integrity of biochemical assays.
Product Gallery
Management Platforms
Quality Control
Strict purity monitoring to ensure a minimum assay of 99.0% for all batches.
Precision Synthesis
Advanced chemical manufacturing processes for consistent molecular weight and purity.
Solubility Testing
Rigorous testing to guarantee colorless and transparent water-solubility.
Stability Tracking
Long-term stability assessments to ensure shelf-life and pH performance.
Logistics Mgmt
Specialized handling for specialty chemicals to prevent contamination during transit.
Compliance Audit
Full adherence to international chemical safety and quality standards.
Investment Return Comparison
| Feature Index | Traditional Buffers | Bicine Buffer |
|---|---|---|
| Metal Chelation | High Interference | Minimal Interference |
| UV Absorbance | Moderate/High | Very Low |
| pH Stability | Variable/Drifts | Highly Stable (7.6-9.0) |
| Cellular Stress | Higher Permeability | Low (Zwitterionic) |
| Enzyme Inhibition | Common | Negligible |
Frequently Asked Questions
Bicine is primarily used as a zwitterionic buffer in protein electrophoresis, chromatography, and enzyme assays to maintain a stable alkaline pH environment.
Bicine exhibits significantly lower metal-chelation properties than Tris, preventing the undesirable removal of metal ions from proteins during crystallography.
No, Bicine is characterized by very low ultraviolet absorbance, making it ideal for applications requiring accurate spectrophotometric analysis.
Bicine is most effective as a buffer in the pH range of 7.6 to 9.0, providing excellent stability in slightly alkaline conditions.
Its zwitterionic structure reduces membrane permeability, which minimizes cellular stress and helps maintain physiological conditions during fractionation.
Yes, Bicine-based buffers are commonly employed in nucleic acid hybridization procedures and various diagnostic kit components.
Need High-Purity Bicine for Your Lab?
Contact Us
Moneide Chemicals
