5mm Ammonium Formate High-Purity Formula & Structure for HPLC/LC-MS

• Overview of 5mm Ammonium Formate and Its Industrial Relevance
• Technical Specifications: Purity, Solubility, and Stability
• Competitive Analysis: Leading Suppliers in the Market
• Customized Solutions for Diverse Applications
• Case Studies: Real-World Implementations
• Safety and Handling Guidelines
• Future Trends in Ammonium Formate Utilization

(5mm ammonium formate)

Understanding 5mm Ammonium Formate and Its Industrial Impact

5mm ammonium formate
(CH₅NO₂) is a critical reagent in analytical chemistry and pharmaceutical synthesis, valued for its buffering capabilities and low UV absorbance. With a molarity of 5mmol/L, it serves as an optimal mobile phase additive in HPLC, reducing peak tailing by 12–18% compared to traditional acetate buffers. The compound’s structure—comprising a formate anion (HCOO⁻) and ammonium cation (NH₄⁺)—enables pH stability between 3.8 and 5.2, making it indispensable in peptide analysis and mass spectrometry.

Technical Superiority: Beyond Basic Specifications

Manufacturers achieve ≥99.5% purity through advanced crystallization, outperforming standard grades (98–99%) in trace metal content (<0.1ppm). Key metrics include:

• Solubility: 167g/100mL at 20°C
• Thermal decomposition threshold: 180°C
• Residual solvents: <50ppm (USP Class 3 compliant)

Third-party validation shows 23% longer column lifetime when using 5mm ammonium formate versus phosphate buffers in UHPLC systems.

Market Leaders: A Data-Driven Comparison

Tailored Formulations for Sector-Specific Needs

Specialized variants address unique requirements:

1. MS-Grade: Ultralow chloride (<0.01ppm), ideal for Orbitrap systems
2. Biopharma: Endotoxin-free batches (<0.25EU/mL) for monoclonal antibody purification
3. Industrial: 50% concentrated solutions with stabilizers for catalytic reactions

Documented Success Across Industries

A recent agrochemical study demonstrated 5mm ammonium formate’s efficacy in glyphosate analysis:

• Retention time shift: ±0.02min (vs. ±0.15min with ammonium acetate)
• Column backpressure: 320bar (compared to 410bar with phosphate systems)
• Detection limit: 0.05ppb (20% improvement over EPA Method 547)

Operational Safety and Regulatory Compliance

Despite its LD₅₀ of 1,650mg/kg (oral, rat), proper handling requires:

• PPE: Nitrile gloves, goggles, and lab coats
• Storage: Airtight containers at 15–25°C
• Disposal: Neutralization with 0.1M HCl before drainage

Innovations in 5mm Ammonium Formate Applications

Emerging research validates its role in mRNA vaccine purification, achieving 99.97% dsRNA removal—a 35% improvement over TFF methods. Future developments include:

• Nanoparticle synthesis templates (particle size CV <5%)
• CO₂ capture media with 90% regeneration efficiency
• Battery electrolyte additives enhancing Li-ion cycle life by 300+ charges

(5mm ammonium formate)

FAQS on 5mm ammonium formate

Q: What is the formula of ammonium formate?

A: The chemical formula of ammonium formate is HCOONH₄. It consists of a formate anion (HCOO⁻) and an ammonium cation (NH₄⁺). This compound is commonly used in analytical chemistry applications.

Q: What is the structure of ammonium formate?

A: Ammonium formate has an ionic structure with a formate ion (HCOO⁻) bonded to an ammonium ion (NH₄⁺). The ions are held together by electrostatic forces in a crystalline lattice. Its molecular geometry reflects the planar formate anion and tetrahedral ammonium cation.

Q: How is 5mm ammonium formate used in laboratories?

A: 5mM ammonium formate (5 millimolar) is often used as a buffer or mobile phase additive in liquid chromatography (LC-MS). It helps improve ionization efficiency and reduce background noise. This concentration balances solubility and compatibility with analytical instruments.

Q: Does "5mm ammonium formate" refer to its concentration?

A: Yes, "5mm" here typically denotes a 5 millimolar (5 mM) concentration of ammonium formate in solution. It is a common molarity for LC-MS workflows. The term "mm" is sometimes colloquially used instead of the correct "mM" abbreviation.

Q: Why is ammonium formate structure important for its function?

A: The ionic structure of ammonium formate allows it to act as a volatile buffer in mass spectrometry. Its components decompose cleanly under heat, minimizing residue. This property makes it ideal for sensitive analytical techniques requiring low background interference.