Moneide Chemicals
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Basic Red 5
Chemical Name |
Basic Red 5 |
Synonyms |
Neutral Red; C.I. 50040; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride |
CAS No. |
553-24-2 |
Molecular formula |
C15H17แคลเอ็น4 |
EINECS No. |
209-035-8 |
Molecular weight |
288.78 |
Molecular Structure |
|
Details |
Appearance: dark green powder pH change range: 6.8(red)~8.0(yellow) Ignition residue(sulfate): 2.0% max. Solublity in Ethanol Passes Test: pass Sensitivity Test: pass Biological dyeing test: pass |
Main Application |
Used as pH indicator; REDOX indicator; Adsorption indicator. |
- Fiber Dyeing: Basic Red 5 is widely used in the textile industry for dyeing various fibers. For cotton fabrics, it can produce a deep and lasting red color, which is popular in the production of clothing, curtains, and bedding. When dyeing wool, it not only imparts a beautiful red color but also helps to enhance the softness and luster of the woolen products. In the case of synthetic fibers like nylon, it can achieve a high - color yield and good colorfastness, making the dyed nylon products suitable for outdoor use and frequent washing.
- Textile Printing: It is also used in textile printing processes. The solubility and color properties of Basic Red 5 allow it to be easily formulated into printing pastes. It can be used to create intricate red - colored patterns on fabrics, adding aesthetic value to textile products. Whether it's for fashion garments, upholstery fabrics, or decorative textiles, Basic Red 5 can bring out detailed and vibrant printed designs.
- Paper Dyeing: In the paper industry, Basic Red 5 is used to color different types of paper products. It can be added to the pulp during the paper - making process to produce red - colored papers for stationery, gift - wrapping papers, and decorative papers. The color is evenly distributed in the paper, resulting in a consistent and attractive red appearance. It can also be used in the printing ink for paper, providing a bright red color for text and graphics on paper - based materials.
- Leather Dyeing: Basic Red 5 can be used to dye leather materials. It penetrates the leather fibers well, giving a uniform and long - lasting red color. This is useful in the production of leather goods such as handbags, shoes, belts, and furniture upholstery. The dyed leather not only looks appealing but also maintains its color during normal use and handling.
- Plastic Coloring: In the plastic and polymer industry, Basic Red 5 can be used to color various plastic products. It can be incorporated into the plastic matrix during the manufacturing process to give plastics a red color. This is applicable to products like plastic toys, packaging materials, and plastic decorative items. The color stability of Basic Red 5 ensures that the plastic products retain their color over time, even under different environmental conditions.
- Basic Red 5 may be harmful if swallowed, inhaled, or absorbed through the skin.
- It may cause skin and eye irritation. Prolonged or repeated exposure may have adverse effects on health.
- Some studies have raised concerns about its potential carcinogenicity, although the exact risks are still under investigation.
- When handling this product, always wear appropriate personal protective equipment, including chemical - resistant gloves, safety goggles, and a lab coat or protective apron.
- Work in a well - ventilated area, preferably under a fume hood, to avoid inhalation of dust or fumes.
- In case of contact with skin, immediately wash the affected area with plenty of water for at least 15 minutes and seek medical advice. If the compound gets into the eyes, rinse the eyes thoroughly with water for at least 15 minutes, lifting the eyelids occasionally, and then seek immediate medical attention.
- Do not eat, drink, or smoke in the area where Basic Red 5 is being used.
- Store Basic Red 5 in a cool, dry place, away from direct sunlight and heat sources.
- Keep it in a tightly sealed container to prevent moisture absorption and contact with air, which could potentially affect its chemical properties and color quality.
- Store it separately from incompatible substances such as strong oxidizing agents and acids to avoid dangerous reactions.
What is basic red 5 dye?
Basic Red 5 is a synthetic cationic dye belonging to the azine class of dyes, chemically classified as a phenazine derivative. This water-soluble dye appears as dark red crystals or powder that produces vibrant red solutions in aqueous media. Its molecular structure contains delocalized positive charges that facilitate strong electrostatic binding to negatively charged cellular components. Commonly known as Safranin O in biological applications, it serves dual purposes as both a biological stain and a redox indicator. The dye maintains excellent stability under various pH conditions and demonstrates good lightfastness properties. Its ability to penetrate cell structures while providing intense coloration makes it valuable for multiple staining protocols in microbiology and histology laboratories.
Which component of the cell is the neutral red dye stain?
Neutral red dye primarily accumulates in acidic compartments of the cell, particularly staining lysosomes and other acidic vesicles. This weak cationic dye penetrates cell membranes and becomes trapped in organelles with low internal pH, where it appears as red-orange inclusions under microscopy. The stain is commonly used to identify viable cells in culture, as it is selectively absorbed by living cells with active proton pumps maintaining acidic intracellular environments. Neutral red also binds to lipofuscin granules and certain secretory vesicles. Its specific accumulation in these acidic components makes it particularly useful for studying endocytosis, lysosomal function, and cellular viability in both mammalian and plant cell systems.
What is the color change of basic red 5 indicator?
Basic Red 5 (Safranin O) exhibits a reversible color change from red to colorless based on its oxidation state, serving as an important redox indicator in biochemical experiments. In its oxidized form, the dye appears intensely red, while reduction converts it to a colorless leuko form. This transition occurs at a redox potential of about -0.289 volts at pH 7, making it suitable for monitoring various biological oxidation-reduction reactions. The color change is particularly useful in bacterial viability tests, where metabolically active cells reduce the dye. The indicator also shows pH-dependent color variations, shifting from orange-red in acidic solutions to deep red in alkaline conditions, though its primary analytical utility remains in redox applications rather than pH indication.