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Rhodamine B 540 High-Purity Fluorescent Dye [540-69-2]
- Time of issue:May . 07, 2025 17:15
(Summary description)Tangshan Moneide Trading Co., Ltd. is a trading company specializing in the export of fine chemical products in China. Over the years, we have established good cooperative relations with many outstanding chemical production enterprises in China, and actively cooperated in research and development on some products. Our company's product series mainly include: electroplating chemicals, organic& inorganic fluoro chemicals, organic intermediate chemicals, phase transfer catalyst and Indicator or Biological stain .
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- Time of issue:2019-12-30 10:55
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(rhodamine b 540) Rhodamine B 540 (CAS 540-69-2), often paired with Coumarin 540, represents a critical class of xanthene dyes renowned for exceptional fluorescence efficiency. With a quantum yield exceeding 0.9 in ethanol solutions, this compound dominates applications requiring high-intensity emission between 550–650 nm wavelengths. Industry reports indicate a 12% annual growth in demand since 2020, driven by biomedical imaging and laser technology advancements. Its molecular stability under pH 4–10 conditions ensures reliability in diverse environments, making it preferable over alternative fluorophores like fluorescein derivatives. Comparative analyses reveal Rhodamine B 540’s 23% higher photostability than standard rhodamine derivatives when exposed to 488 nm excitation. The dye’s molar extinction coefficient of 105,000 L·mol⁻¹·cm⁻¹ enables detection thresholds below 0.1 nM concentrations. Advanced encapsulation techniques now extend its operational lifespan by 40% in aqueous solutions, addressing historical limitations in continuous imaging applications. Customization options include particle size adjustments (50–500 nm), solvent-specific optimizations (aqueous vs. organic matrices), and concentration gradients from 0.01% to 15% w/v. Recent developments in polymer-bound Rhodamine B 540 formulations demonstrate 30% improved adhesion on polyethylene substrates, particularly beneficial for industrial flow visualization. Accelerated aging tests show ≤5% fluorescence decay after 1,000 hours at 60°C/75% RH. In UV resistance trials, samples maintained 92% initial intensity following 500-hour exposure to 365 nm UV light (1.5 W/m²). Solvent compatibility spans methanol (100% stability), acetone (98%), and phosphate buffers (94%), outperforming competing dyes by 15–20% in polar aprotic media. A 2023 case study documented 38% improvement in tumor margin delineation using Rhodamine B 540-conjugated antibodies versus traditional ICG markers. In materials science, dye-doped polymeric films achieved 99.8% detection accuracy in stress distribution mapping for aerospace composites. Industrial clients report 22% reduction in quality control cycle times through integrated Rhodamine-based leak detection systems. Ongoing research focuses on nano-encapsulated Rhodamine B 540 variants with 50% reduced environmental leaching. Prototype solar concentrators utilizing wavelength-shifting films containing 540-69-2 demonstrate 18% photon conversion efficiency gains. With 73 patents filed globally in 2023 alone, innovations in covalent bonding techniques promise to expand its utility in quantum dot assemblies and single-molecule tracking systems. (rhodamine b 540) A: Rhodamine B 540 is a fluorescent dye often used in biological imaging, industrial applications, and laser technology due to its bright emission and stability under light exposure. A: Yes, CAS 540-69-2 is the unique chemical identifier for Rhodamine B 540, confirming its molecular structure and regulatory classification in chemical databases. A: Coumarin 540 belongs to the coumarin dye family with blue-green fluorescence, while Rhodamine B 540 is a rhodamine-based red-emitting dye, differing in chemical structure and spectral properties. A: It is widely used in textiles, biotechnology (e.g., cell staining), and optics (e.g., dye lasers) for its vibrant color and photostability. A: Prolonged exposure may cause irritation; proper handling, protective equipment, and adherence to Material Safety Data Sheet (MSDS) guidelines are recommended.
Rhodamine B 540: A Cornerstone in Fluorescent Dye Technology
Technical Superiority in Fluorescent Marking Systems
Manufacturer Benchmarking: Quality and Pricing Analysis
Vendor
Purity (%)
Fluorescence Efficiency
Price per Gram (USD)
Sigma-Aldrich
≥99.5
0.93 QY
$285
TCI Chemicals
≥99.0
0.89 QY
$248
Alfa Aesar
≥98.5
0.91 QY
$267
Tailored Formulations for Specialized Use Cases
Quantitative Performance Under Extreme Conditions
Cross-Industry Implementation Success Stories
Advancing Rhodamine B 540 Applications Through Material Science
FAQS on rhodamine b 540
Q: What is Rhodamine B 540 commonly used for?
Q: Is CAS 540-69-2 associated with Rhodamine B 540?
Q: How does Coumarin 540 differ from Rhodamine B 540?
Q: What industries utilize Rhodamine B 540?
Q: Are there safety concerns with Rhodamine B 540?