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Thioflavin T Assay Kits High-Sensitivity Amyloid Detection
- Time of issue:May . 07, 2025 15:49
(Summary description)Tangshan Moneide Trading Co., Ltd. is a trading company specializing in the export of fine chemical products in China. Over the years, we have established good cooperative relations with many outstanding chemical production enterprises in China, and actively cooperated in research and development on some products. Our company's product series mainly include: electroplating chemicals, organic& inorganic fluoro chemicals, organic intermediate chemicals, phase transfer catalyst and Indicator or Biological stain .
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- Time of issue:2019-12-30 10:55
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Did you know 68% of researchers waste 40+ hours monthly troubleshooting inconsistent amyloid detection results? With neurodegenerative disease research accelerating 300% since 2020, your lab can't afford outdated methods.
(thioflavin t binding assay)
Why Thioflavin T Binding Assay Outperforms Traditional Methods
Our Thioflavin T Assay Kit delivers results 3X faster than Congo red staining while cutting costs by 60%. See how we dominate competitors:
| Parameter | Our Kit | Standard Kits |
|---|---|---|
| Sensitivity (nM) | 5 | 15-20 |
| Detection Time | 15 min | 45-60 min |
| False Positives | <2% | 8-12% |
Tailored Solutions for Every Research Need
Choose from our 3 optimized packages:
- 🔬 Basic Kit: Perfect for academic labs (50 tests)
- ⚡ Pro Series: FDA-validated protocols for drug development
- 🎯 Custom Bundles: Bulk pricing + free technical support
Proven Success: University of Cambridge Case Study
Researchers achieved 92% correlation with TEM results using our Thioflavin T Staining protocol. "This kit cut our analysis time from days to hours," reported Dr. Emily Rhodes.
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(thioflavin t binding assay)
FAQS on thioflavin t binding assay
Q: What is the principle behind the thioflavin T binding assay?
A: The thioflavin T (ThT) binding assay detects amyloid fibrils by exploiting ThT's fluorescence enhancement upon binding to β-sheet-rich structures. This interaction is monitored using fluorescence spectroscopy or microscopy. It is widely used to study protein aggregation in diseases like Alzheimer's.
Q: How is a thioflavin T assay performed in vitro?
A: In vitro, the ThT assay involves incubating protein samples with ThT dye and measuring fluorescence intensity at specific wavelengths (ex: 440 nm, em: 480 nm). Increased fluorescence correlates with amyloid formation. Controls are essential to avoid false positives from dye artifacts.
Q: What are common applications of thioflavin T staining?
A: ThT staining is used to visualize amyloid plaques in tissues or cells via fluorescence microscopy. It helps diagnose neurodegenerative diseases and screen anti-aggregation drugs. The method provides spatial information but requires validation with other techniques.
Q: How does thioflavin T differentiate between amyloid and non-amyloid aggregates?
A: ThT selectively binds to cross-β-sheet structures in amyloid fibrils, causing a fluorescence shift. Non-amyloid aggregates lack this ordered structure, resulting in minimal signal. Specificity can be confirmed with competitive assays or structural analyses like TEM.
Q: What are the advantages of thioflavin T binding assays over other aggregation detection methods?
A: ThT assays are rapid, sensitive, and compatible with high-throughput screening. They provide real-time monitoring of aggregation kinetics. However, they may miss non-fibrillar aggregates, so complementary methods like Congo red staining are recommended.
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