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o-Cresolphthalein Complexone
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Chemical Name |
o-Cresolphthalein Complexone |
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Synonyms |
o-Cresolphthalexon; Phthalein Complexon; o-Cresolphthaleindi-(methyliminodi-acetic acid); 3',3''-Bis[[bis(carboxymethyl)amino]methyl]-5', 5''-dimethylphenolphthalein |
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Molecular formula |
C32H32N2O12 |
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CAS No. |
2411-89-4 |
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EINECS No. |
219-318-8 |
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Molecular weight |
636.61 |
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Molecular Structure |
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Details |
Appearance: White or light pink crystalline powder Assay: 80% min. Sensitivity: Passes test Solubility in water: Passes test Sulfated ash: 1.0% max. Dissociative toluene phenolphthalein: Passes test Organic impurity: Passes test Solubility: solve in alcohol,aether and acetic acid, difficult solve in benzene Packing: 25kg/ fibre drum |
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Main Application |
Complexation indicator |
- Metal Ion Detection and Quantification: In analytical chemistry, o - Cresolphthalein Complexone is widely used for the detection and quantification of metal ions. For example, it can be used in complexometric titrations to determine the concentration of calcium and magnesium ions in water samples. In these titrations, the complexone forms a colored complex with the metal ions, and the endpoint of the titration is indicated by a change in color. This method is highly accurate and reliable for determining the hardness of water.
- Quality Control in Industrial Processes: It can also be used for quality control in industrial processes that involve metal ions. In the production of pharmaceuticals, food products, and electronic materials, the presence of certain metal ions can affect the quality and performance of the final product. o - Cresolphthalein Complexone can be used to detect and control the levels of these metal ions, ensuring that the products meet the required quality standards.
- Biochemical Assays: In biochemical assays, o - Cresolphthalein Complexone can be used to measure the concentration of metal ions in biological samples such as blood, urine, and tissue extracts. For example, it can be used to determine the calcium ion concentration in blood serum, which is an important parameter in the diagnosis of various diseases. The chelating ability of the complexone allows it to selectively bind to calcium ions, and the resulting color change can be measured spectrophotometrically.
- Cell Culture and Biotechnology: In cell culture and biotechnology applications, o - Cresolphthalein Complexone can be used to control the concentration of metal ions in cell culture media. Metal ions such as calcium and magnesium are essential for cell growth and function, but excessive or insufficient levels can be harmful. The complexone can be used to adjust the metal ion concentration in the media, providing an optimal environment for cell growth and proliferation.
- Water Quality Analysis: In environmental monitoring, o - Cresolphthalein Complexone is used for water quality analysis. It can be used to determine the hardness of water, which is an important parameter for assessing the quality of drinking water and industrial water supplies. The complexone reacts with calcium and magnesium ions in water to form a colored complex, and the intensity of the color is proportional to the concentration of the metal ions. This method is simple, rapid, and cost - effective for water quality monitoring.
- Soil Analysis: It can also be used in soil analysis to determine the availability of metal ions in soil. The chelating ability of the complexone allows it to extract metal ions from soil samples, and the resulting complexes can be analyzed to determine the concentration and speciation of the metal ions. This information is useful for understanding the fertility of soil and the potential for metal ion contamination.
- o - Cresolphthalein Complexone is harmful if swallowed, inhaled, or absorbed through the skin.
- It may cause skin and eye irritation. Prolonged or repeated exposure may have adverse effects on health.
- Contact with strong oxidizing agents can lead to dangerous reactions.
- When handling this product, always wear appropriate personal protective equipment, including chemical - resistant gloves, safety goggles, and a lab coat or protective apron.
- Work in a well - ventilated area, preferably under a fume hood, to avoid inhalation of dust or fumes.
- In case of contact with skin, immediately wash the affected area with plenty of water for at least 15 minutes and seek medical advice. If the compound gets into the eyes, rinse the eyes thoroughly with water for at least 15 minutes, lifting the eyelids occasionally, and then seek immediate medical attention.
- Do not eat, drink, or smoke in the area where o - Cresolphthalein Complexone is being used.
- Store o - Cresolphthalein Complexone in a cool, dry place, away from direct sunlight and heat sources.
- Keep it in a tightly sealed container to prevent moisture absorption and contact with air, which could potentially affect its chemical properties.
- Store it separately from incompatible substances such as strong oxidizing agents and acids to avoid dangerous reactions.
What is o-cresolphthalein complexone?
o-Cresolphthalein complexone is a synthetic polyaminocarboxylic acid derivative created by modifying o-cresolphthalein with iminodiacetic acid groups. This chelating agent forms the basis of a highly sensitive colorimetric method for calcium determination, where it produces a distinctive purple-red complex with calcium ions in alkaline solutions. The compound retains the chromophoric properties of its parent o-cresolphthalein while gaining enhanced metal-binding capacity through its additional complexone functional groups. Its molecular architecture combines the pH-sensitive dye characteristics with multiple metal-coordinating sites, creating a bifunctional reagent capable of both selective metal ion recognition and vivid color response. This dual functionality makes it uniquely valuable for analytical applications requiring both metal specificity and visual detection.
O-Cresolphthalein Complexone Application
The primary application of o-cresolphthalein complexone is in clinical chemistry for precise measurement of serum and urinary calcium levels. When mixed with calcium ions in alkaline buffer (pH ~10-12), it forms a stable 1:1 complex with intense absorbance at 570-580 nm, enabling accurate spectrophotometric quantification. This method has become the gold standard for automated clinical analyzers due to its exceptional specificity for calcium over other cations. The reagent also finds use in water hardness testing and quality control of calcium-containing pharmaceuticals. Recent adaptations include its incorporation into test strips for point-of-care calcium monitoring and as a detection reagent in microfluidic analytical devices, demonstrating its continued relevance in modern diagnostic technologies.
What is the feature of o-cresolphthalein complexone?
o-Cresolphthalein complexone's most notable feature is its exceptional selectivity for calcium ions in the presence of magnesium, achieving about 1000:1 discrimination. The compound exhibits high molar absorptivity (ε≈30,000 L·mol⁻¹·cm⁻¹) for its calcium complex, enabling sensitive detection at micromolar concentrations. Its color transition is remarkably stable, resisting interference from common serum components like proteins and phospholipids. The complexone modification provides eight coordination sites that envelop the calcium ion, creating both the selectivity and intense chromogenic response. These combined properties - high sensitivity, excellent specificity, and stable color development - make it superior to earlier calcium detection methods, particularly for clinical applications where accuracy and reproducibility are critical. The reagent remains stable in solution for extended periods when properly stored.
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