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High Purity Ponceau S CAS 6226795 China Factory Suppliers
Ponceau S is a high-performance, acidic sulfonated azo dye specifically engineered for precision biological staining. As a water-soluble compound within the Ponceau dye family, it is characterized by a sophisticated molecular design featuring multiple sulfonate groups. This structure enhances its solubility and provides critical negative charges, allowing for efficient electrostatic interactions with target proteins, resulting in vibrant red coloration for high-contrast visualization.
Primarily utilized in advanced protein biochemistry, Ponceau S serves as an indispensable tool for Western blotting and electrophoresis. Its unique ability to provide reversible staining allows researchers to verify protein transfer efficiency and distribution on membranes or gels without causing permanent modification. This ensures that the antigenicity of the sample remains intact for subsequent immunodetection, making it a superior choice for rigorous laboratory quality control.
Detailed Parameters
| Chemical Name | Ponceau S | CAS No. | 6226-79-5 |
|---|---|---|---|
| Molecular Formula | C22H12N4Na4O13S4 | EINECS No. | 228-319-2 |
| Molecular Weight | 760.57 | Appearance | Brown powder |
| Color (Water) | Red | Color (Sulfuric Acid) | Blue |
| Biological Test | Pass Test | Package | 25kg/fibre drum |
Key Advantages
Fully Reversible
Enables temporary visualization without permanent protein modification, preserving samples for downstream analysis.
Rapid Results
Provides near-instant protein visualization within 1-2 minutes, significantly accelerating laboratory workflows.
High Sensitivity
Capable of detecting nanogram quantities of protein, ensuring precision in low-concentration samples.
Preserves Antigenicity
Does not require fixation, allowing antibodies to bind effectively after the dye is washed away.
Superior Solubility
Excellent water-solubility allows for easy preparation of stable solutions in aqueous buffers.
Cost-Effective
Combines professional-grade performance with low operational costs for routine lab applications.
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Management Platforms
Quality Control
Strict adherence to purity standards for pharmaceutical and biological intermediates.
Batch Traceability
Comprehensive tracking systems for every production lot of specialty chemicals.
Compliance Monitoring
Regular audits ensuring products meet international safety and chemical standards.
Supply Chain Optimization
Efficient logistics management to ensure timely delivery of sensitive chemical agents.
Storage Standards
Climate-controlled warehousing to maintain the stability of organic intermediates.
Technical Support
Expert guidance on dye application and protocol optimization for researchers.
Investment Return Comparison
| Metric | Standard Protein Stains | Ponceau S Solution |
|---|---|---|
| Staining Time | 15 - 60 Minutes | 1 - 2 Minutes |
| Reversibility | Permanent / Hard to Remove | Highly Reversible |
| Antigen Preservation | May hinder antibody binding | Full preservation |
| Workflow Impact | Requires separate blot | Single-blot verification |
| Operational Cost | Moderate to High | Low / High Efficiency |
Frequently Asked Questions
Unlike permanent stains, Ponceau S is reversible. It allows for the visualization of protein bands on a membrane and can be easily washed off, enabling subsequent antibody probing without interfering with the results.
It is used as a rapid assessment tool after the transfer of proteins from a gel to a membrane. By staining the membrane, researchers can confirm the transfer efficiency and locate the protein bands before proceeding to immunodetection.
Yes, it is widely compatible with standard membranes used in electrophoresis, such as PVDF and nitrocellulose, providing clear and stable visualization across various substrates.
Ponceau S should be stored in a cool, dry place in tightly sealed containers, typically provided in 25kg fibre drums to ensure stability and prevent contamination.
While its primary use is as a biological stain, it is also applied in the histological staining of connective tissues and serves as a food colorant in specific authorized regions.
Because of its water-soluble nature, Ponceau S can be removed with simple washes using water or standard buffer solutions, returning the membrane to its original state for the next step of the experiment.
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